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Fig.1 Collapse and Encapsulation of DNA
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Molecular Manipulation in Electro-Spun Scaffold for Gene Delivery(Chu):
Laser light scattering and TEM were used to characterize plasmid DNA, first collapsed in a poor solvent mixture of 94% DMF and 6% 1XTE buffer with a volume contraction of ~ 103. Then, the collapsed DNA was encapsulated by using a tri-block copolymer of lactide (L) and ethylene glycol (E), as shown in Figure 1 with data listed in Table 1.
Electro-spinning of poly(lactide-glycolide) with encapsulated in the same solvent mixture could form a nano-fibrous scaffold containing bioactive plasmid DNA, as shown in Figure 2.
The scaffold has the suitable morphology that will enable endogenous cells to migrate into it, adhere, proliferate, and initiate new matrix/osteoid production.
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