HYDROGENASES 97
 

Scientific Programme

 

Saturday July 12 1997
 

14h00-17h00 Registration - Poster mounting - Poster browsing

 

17h30-17h35	Opening of the Conference

 

17h35-17h40	COST presentation by K.L. Kovacs

 

17h40-18h10	Session I
`		Chairperson: P.M. Vignais

		H. G. Schlegel: Some history...on hydrogen and hydrogenases

 

18h30-20h30	Get-together party





Sunday July 13 1997

8h30-10h00	Session II:
		Chairperson: H.G. Schlegel

8h30		S. P. J. Albracht (overview): Low-spin iron: the heart of hydrogenases	

9h15		B. Friedrich (overview): NiFe-hydrogenase: Complex biogenesis and structural/functional 							 variations of a redox catalyst.

 

10h00-10h30	Coffee break

 

10h30-12h10	Session III: Protein Structure - Active site I
		Chairperson: T. Yagi

10h30		J. Fontecilla-Camps: Structural bases for the catalytic mechanism of [NiFe]hydrogenases

10h55		 M. J. Maroney: Investigations of the structure and function of the nickel site in Chromatium 					 vinosum hydrogenase

11h20		 Y. Higuchi: A plausible assignment of diatomic ligands to Fe atom in Ni-Fe active center in 					hydrogenase at 1.8 Å resolution

11h45		 W. Lubitz: Single crystal EPR and ENDOR of the active site of the [NiFe]hydrogenase from 					Desulfovibrio vulgaris

 

12h10-14h00	Buffet

 

14h00-16h00	Poster Session I
16h- 16h30	Coffee break



16h30-18h35	Session IV: Active site II
		Chairperson: P. Bertrand

16h30		J. J. G. Moura: Spectroscopic evidence for the presence of iron in the novel heterobinuclear site of 					 nickel hydrogenases

16h55		B. Guigliarelli: Electronic and magnetic properties of the Ni-Fe site of hydrogenases. Implications 					 for the catalytic mechanism.

17h20		V. M. Fernandez: FTIR-spectroelectrochemistry of [NiFe]hydrogenases.



17h45-17h55	Break



17h55-		E. Munck: Mossbauer studies of [NiFe]hydrogenase from Chromatium vinosum.

18h20-		W. R. Hagen: A new model for the structure, oxidation states, and reductive activation of the 					 catalytic center in Fe-hydrogenase

18h45		R. Cammack: Insights into the structure and mechanism of D. gigas hydrogenase from EPR and 					 ENDOR spectroscopy



19h30		Dinner





Monday July 14 1997

8h30-10h25	Session V: Genetics & Biosynthesis I
		Chairperson: B. Friedrich

8h30-		A. Böck: Biosynthesis of hydrogenases in E. coli

9h10-		R. J. Maier: Hydrogenase maturation in Bradyrhizobium japonicum

9h35-		T. Ruiz-Argüeso: Hydrogenase gene expression in Rhizobium leguminosarum bv. viciae is 					 controlled by symbiotic regulatory genes

 

10h00		B.Toussaint: Regulation of hydrogenase synthesis in the photosynthetic bacterium Rhodobacter 					 capsulatus

 

10h25-11h00	Coffee break

 

11h00-12h40	Session VI: Genetics & Biosynthesis II
		Chairperson: J.D. Wall

11h00		J.D. Wall: Role and regulation of [NiFe]hydrogenase in Desulfovibrio desulfuricans strain G20.

11h25		A. Klein: Regulation of the expression of gene groups encoding selenium-free NiFe-hydrogenases 				 in Methanococcus voltae.

11h50		K. Fiebig: Analysis of an operon-like gene cluster from Methanosarcina barkeri encoding a 				 coenzyme F420-reactive [NiFe]hydrogenase.

12h15		K. Kovacs: Studies on [NiFe]hydrogenases from phototrophic, methanotrophic, and 						hyperthermophilic microorganisms.

 

12h40-14h00	Buffet

 

14h00-16h00	Poster Session II (15h30-16h: Coffee break)
 

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6h00-17h30	Session VII: Genetics & Biosynthesis III
		Chairperson: D. Boxer

16h00		E. Schwartz: Functional organization of the Alcaligenes eutrophus hydrogenase genes

16h15		S. Andrews: Hydrogenase-4 of E. coli.

16h30		L.-F. Wu: Translocation of hydrogenase and TMAO reductase of Escherichia coli

17h00-		D. Dean: Biosynthesis of iron-sulfur clusters.

 

17h30-18h15h	Round-Table I (including Discussion of Posters): Genetics & Biosynthesis
		Discussion organizers: M.A. Mandrand; R. L. Robson.

		

18h15h-19h	Round-Table II: Structure and Function of hydrogenases
		Discussion organizer: R. Thauer

		

19h30		Dinner

 

 

Tuesday July 15 1997
 

8h30 -10h		Session VIII: Synthetic models
		Chairperson: J. Jordanov

8h30		P. Mascharak: Reactions of hydrogen and CO with nickel complexes of N/S/Se-donor ligands; 					 relevance to [NiFe]hydrogenase.

8h55		M. Millar: Synthetic Analogs for the Ni-Fe Center in Hydrogenases

9h20		M. Darensbourg: Hetero bimetallic complexes in model studies for [NiFe]hydrogenase

9h45		R.K. Henderson: Modelling nickel hydrogenases.

10h00 		M. Schröder: Routes to models of [NiFe]hydrogenase via compartmental macrocyclic chemistry.

 

10h15-10h45	Coffee break

 

10h45		B. Chaudret: Dihydrogen complexes and proton transfer in hydride complexes: a relation with 					 hydrogenases?

11h10		K. Bagley: Infrared spectroscopic study of the spin coupled state in oxidized hydrogenase in 				 Chromatium vinosum.

11h35		A. Pierik: Recent developments in FTIR on [NiFe] and Fe-only hydrogenases.

 

11h50-12h00	Break

 

12h00-13h00 	Session IX Active site III
		Chairperson: W.R. Hagen



12h00		E. Garcin: Crystallographic studies of the hydrogenase from Desulfomicrobium baculatum

				 DSM 1743 (D. baculatum).

12h15-		Y. Montet: Crystallographic studies of D. fructusovorans hydrogenase. Gas access to the active 				 site probed by X-ray crystallography and by molecular dynamics.

12h30		O. Sorgenfrei: Differences in the electronic structure around Ni in oxidized and reduced selenium-					 containing hydrogenases from Methanococcus voltae.

12h45		N. Zorin: Catalytic properties of Thiocapsa roseopersicina hydrogenase 1. Inhibition by 					 iodoacetamide and acetylene.

 

13h -14h		Picnic

 

14h -18h		Excursion

 

19h30-23h	Dinner at Aix-Marlioz and return to Albertville

 

 

Wednesday July 16 1997

 

8h30- 10h00	Session X: Biological Functions and Applications I
		Chairperson: P. Soucaille

8h30		H. Bothe: Molecular analysis of hydrogen metabolism in cyanobacteria.	

8h55		P. Lindblad: Nostoc PCC 73102 and H2: knowledge, research and biotechnological challenges.

9h20		R. Schulz: Hydrogenases of the green alga Scenedesmus and the cyanobacterium Synechocystis: 				 Hypotheses for their coupling to respiration and photosynthesis.

9h45		L. Seefeldt: The role of nucleotides in nitrogenase catalysis

 

10h00-11h15	Poster Session III ( 10h30-11h Coffee break)
 

11h15-12h00	Session XI: Biological Functions and Applications II
		Chairperson: K.L. Kovacs

11h15		M.W.W. Adams: Biological functions and applications of hyperthermophilic hydrogenases.

11h40		M. Rousset: Development of genetic tools in Desulfovibrio fructosovorans [NiFe]hydrogenase 					catalytic properties.

11h55		A. Tsygankov: Stimulation of hydrogen uptake in cells of Anabaena variabilis by oxygen.

12h10		G. Mura. Pyrococcus furiosus hydrogenase and titanium dioxide: environmental application to 				 lactose and dairy waste photodegradation.

 

12h25-14h00	Buffet

 

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4h30-16h00		Session XII: Environmental and Energy-related aspects
		Chairperson: K. Rao

14h30		D.O. Hall: When will photobiological production of H2 become economic?

15h00		P. Weaver: Variant O2-resistant hydrogenases from photosynthetic bacteria oxidizing CO -

				 Bioproduction of neat H2 from thermally gasified biomass

15h30		J. Miyake: Biological hydrogen production R&D of RITE BioHydrogen project, a MITI's 				 program of Japan.

 

16h00-16h30	Coffee-break

 

16h30-17h30	Round-Table III (including Discussion of Posters) (Biotechnological Applications)
		Discussion organizer: D.O. Hall

 

17h30-18h30	Round-Table IV (Concluding Remarks)

		Discussion organizer: R. Cammack

 

18h30-18h35	Closing of the Conference

 

20h30		Banquet

 

 

Thursday July 17 1997
 

7h00	Breakfast

 

	Departure